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KMID : 0617219960070010009
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Duksung Bulletin Phamaceutical Sciences
1996 Volume.7 No. 1 p.9 ~ p.15
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Metabolism of Epidermal Growth Factor after Topical Application
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Cho Ae-Ri
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Abstract
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The metabolic property of Epidermal Growth Factor, EGF, in the second degree burn skin was studied with the freshly prepared skin homogenates and was compared with the one of normal skin.
TLC-Autoradioluminascent graphy, TLC-ARLG, was employed to identify the metabolites of EGF in the skin. In TLC-ARLG study, a new radiation image sensor, a Imaging Plate which is tens to hundreds times more sensitive than previous methods using x-ray film in detecting radio-active specimens was employed.
TLC-ARLG study showed that ^125I-EGF was not significantly metabolized in in vitro metabolism study with skin homogenate. Up to 30 min incubation time, 84% of EGF remained as stable and 4% of EGF was metabolized into a peptidic metabolite and 2% of EGF was metabolized into another metabolite.
And about 10% of ^125I-EGF was presented as a free ^125I.
In the second degree burn skin(100℃, 5 sec burn), no metabolism of EGF was observed indicating burn skin had lost some of the enzymatic capacity of the skin. Our experimental results suggest that EGF, a spherical type polypeptide, does not undergo the significant proteolytic degradation in the skin.
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KEYWORD
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